Volume No. 7 Issue No.: 4 Page No.: 1340-1344 April-June 2013

 

OPTIMIZATION AND PRODUCTION OF ?-GLUCOSIDA-SE FROM THE PLANT PATHOGENIC FUNGI Bipolaris oryzae UNDER SOLID-STATE CULTIVATION OF VETIVER GRASS USING RESPONSE SURFACE METHODOLOGY

 

Samutnavee W., Chuntranuluck S. 1, Kosugi A. 2, Thanapase W.  and Vaithanomsat P.*3

1.   Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok  
             (THAILAND)
       2.   The Japan International Research Center for Agricultural Sciences (JIRCAS) (JAPAN)
       3.   Kasetsart Agricultural and Agro-Industrial Product Improvement Institute (KAPI),   
             Kasetsart University, Bangkok (THAILAND)

 

Received on : November 10, 2012

 

ABSTRACT

 

Solid-state cultivation of vetiver grass using Bipolaris oryzae was performed. L-asparagine concentration, initial pH and raw material-to-moisture were optimized in culture flasks using response surface methodology with respect to specific activity of β-glucosidase in the culture. L-asparagine concentration at 2.70 g l-1, pH of 6.3, and raw material-to-moisture 1: 3.35 (w/v) were found to be the most optimum. Optimized parameters were used for laboratory scale-up in static tray fermenter. The maximum specific activity of β-glucosidase at 1259.72 U mg-protein-1 was obtained after 7 day incubation in static tray fermenter.

 

Keywords : β-glucosidase, Plant pathogenic fungus, Vetiver grass, Response surface methodology, Optimized parameters

 

 

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