Volume No. 7 Issue No.: 3 Page No.: 1246-1253 Jan. - Mar.2013

 

PHYSIOLOGICAL AND BIOCHEMICAL RESPONSES OF A MALAYSIAN RED ALGA, Gracilaria manilaensis TREATED WITH COPPER, LEAD AND MERCURY

 

Zakeri Hazlina Ahamad* and Shuib Nor Shuhanija

Department of Biological Sciences, Faculty of Science and Technology, Universiti Malaysia Terengganu (UMT), Kuala Terengganu (MALAYSIA)

 

Received on : August 10, 2012

 

ABSTRACT

 

The relative effects of three heavy metals, copper (Cu), lead (Pb) and mercury (Hg) exposure on the photosynthetic quantum yield (Fv/Fm), ion leakage and activity of two antioxidative enzymes, catalase (CAT) and Ascorbate Peroxidase (APX) on a Malaysian red alga, Gracilaria manilaensis were investigated. Of the three metals, Hg was observed to affect the alga the most. There was about 89% reduction in the Fv/Fm and 63% increase in ion leakage of the alga in the presence of Hg. In contrast, there was an increased in the activity of both the antioxidative enzymes. CAT was increased to 11 U/mg Total Soluble Proteins (TSP) as compared to 0.4 U/mg TSP in the control while APX was increased to 0.12 U/mg TSP as compared to 0.01 U/mg TSP in the control. Both Cu and Pb did not show any significant changes in the ion leakage of the alga. However, there was a 17% and 12% reduction in Fv/Fm of the alga in Cu and Pb, respectively. CAT and APX in Cu were increased to 1.8 and 1.2 U/mg TSP as compared to 0.4 and 0.02 U/mg TSP in control, respectively. Pb on the other hand, increased the activity of CAT from 0.5 U/mg TSP to 2.3 U/mg TSP and APX from 0.01 U/mg TSP to 0.3 U/mg TSP

 

Keywords : i>Gracilaria manilaensis, Fv/Fm, ion leakage, Antioxidative enzymes, Heavy metals effect

 

 

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