Volume No. 7 Issue No.: 3 Page No.: 1216-1223 Jan. - Mar.2013

 

In Vitro PROPAGATION OF Bambusa tulda : AN IMPORTANT PLANT FOR BETTER ENVIRONMENT

 

Sharma Pratibha* and Sarma K. P.

Department of Environmental Science, Tezpur University, Tezpur, Assam (INDIA)

 

Received on : July 21, 2012

 

ABSTRACT

 

An efficient micro propagation protocol was achieved from nodal explants from field grown culms of Bambusa tulda. Explants were used to induce multiple shoots on Murashige and Skoog medium supplemented with auxins and cytokinins. In vitro auxiliary shoot formation was highest in Murashige and Skoog basal medium supplemented with 1.0 mg/l 6-Benzyle Adenine (BA). Subcultures of shoots from clumps were continued several times for maintaining a stock of mother culture. Clumps of at least 3 shoots were used for root induction in MS medium with Indole-3- Acetic Acid (IAA), Indole-3- Butyric Acid (IBA) and naphthalene acetic acid (NAA). Response of rooting was found more in 5.0 mg/l naphthalene acetic acid. Rooted plantlets were successfully acclimatized in green house for 20 to 25 days and then were transferred to the natural field condition. The survival rate was recorded 100 percent in field condition. To the best of our knowledge this is the first report on in vitro generation of Bambusa tulda from mature field grown auxiliary bud in commercial scale in North-East India. In this paper, a continuous mass multiplication protocol of B. tulda was described, which is cost effective, easy to raise, economic to adopt, easy to transport for selling purpose.

 

Keywords : Multiple shoots induction, Nodal explants, Auxiliary bud, Plantlets regeneration, Bambusa tulda

 

 

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