Volume No. 7 Issue No.: 2A Page No.: 1021-1025 Oct. - Dec. 2012

 

TWO STEP SYNTHESIS AND BIO-PHYSICAL CHARAC-TERISATION OF SILVER NANOPARTICLES USING GREEN APPROACH

 

Mangrola¬ M. H.¬¬*, Joshi V. G.1, Dudhagara P. R.2 and Parmar B. H.3

1.Department of Physics, Veer Narmad South Gujarat University, Surat, Gujarat (INDIA)
2.Department of Biotechnology, Veer Narmad South Gujarat University, Surat, Gujarat (INDIA)
3.Department of Physics, Government Science College, Valod, Gujarat (INDIA)

 

Received on : July 03, 2012

 

ABSTRACT

 

Various schemes are exist and are being used for the synthesis of the silver nanoparticles due to its multiple vital applications however synthesis of nanoparticles may not safe for environment and may generate the hazardous waste. This fact creates the prompt need of the green method for the synthesis of silver nanoparticles. In the present study the eco-friendly approach has been applied for synthesis of silver nanoparticles. Aqueous extract of the Parthenium leaf and 0.5 mM AgNO3 were used for the synthesis. Synthesis reaction was started at 100 °C temperature using different volume of the leaf extract and 0.5 mM AgNO3. Ratio of leaf extract: AgNO3 were 10:1 (SA), 10:0.5(SB), 10:2 (SC). Maximum absorbance was reported at 440 nm in 10:1 (SA) ratio followed by 10:2 (SC) and 10:0.5(SB). Nanoparticles formation was also detected during the synthesis due to the colour changes in the mixture. An antibacterial property was evaluated using six different Gram negative and Gram positive pathogenic bacteria by cup method on bacteriological media. Significant zone of inhibition was found against Staphylococcus aureus, Salmonella typhi, Escherichia coli, Pseudomona aerogens, Protease vulgaris and Bacillus subtilis indicating the antibacterial potential of the synthesised silver nanoparticles. So such scheme for the synthesis of nanoparticles has a great impending applications at large scale.

 

Keywords : Silver nanoparticles, Parthenium, Antibacterial, Pseudomona aerogens, Synthesis reaction

 

 

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