Volume No. 7 Issue No.: 2A Page No.: 1009-1015 Oct. - Dec. 2012




Das Debadatta* and Kaur Rajdeep

Department of Biotechnology and Medical Engineering, National Institute of Technology, Rourkela (INDIA


Received on : September 10, 2012




Phenol is one of the most hazardous pollutants present in industrial effluent. It is of considerable health concern even at low concentration. Phenolic waste water produced by the various processes also cause a major impact on the aquatic ecosystem. The increasing toxicity of phenol has become an alarming reason for its treatment. There are many conventional methods (chemical and physical) for the treatment of phenolic wastes. But they suffer from the problem of secondary effluent and hazardous by-products. Therefore in recent years much emphasis is being given to develop simple and reliable methods which can evaluate the degree of pollution. Biological treatment using microorganisms has proved to be the most attractive and successful process in the current scenario. This can completely degrade the phenol with no harmful end product. The present study was undertaken to check the efficiency of microorganisms like Pseudomonas putida NCIM 2650, Pseudomonas aeruginosa NCIM 2074 and mixed culture (collected from industrial effluent) for the biodegradation of phenol. The whole process was carried out in shake flask batch culture using different concentrations of phenol varying from 100-1250 ppm. Results suggest that under optimum conditions of temperature i.e. 30°C, pH 7.0 and 150 rpm Pseudomonas aeruginosa proved to be an efficient host for phenol degradation with a degrading potential of 1250ppm


Keywords : Biodegradation, Phenol, Pseudomonas putida, Pseudomonas aeruginosa and Mixed culture, Batch culture