Volume No. 7 Issue No.: 2A Page No.: 995-1001 Oct. - Dec. 2012

 

DEGRADATION OF ORGANOPHOSPHATE AND ORGA-NOCHLORINE PESTICIDES IN LIQUID CULTURE BY MARINE ISOLATE Nocardiopsis SPECIES AND ITS BIOPROSPECTIVES

 

Dudhagara Pravin*1,2, Bhalani Sandip1, Bhatt Shreyash2 and Ghelani Anjana3

1. Department of Biotechnology, Veer Narmad South Gujarat University, Udhana-Magdalla Road, Surat (INDIA)
2. Department of Life Sciences, Hemchandracharya North Gujarat University, Patan, Gujarat (INDIA)
3. ShreeRamkrishna Institute of Computer education and Applied Sciences, MTB college Campus, Athawalines, Surat (INDIA)

 

Received on : September 15, 2012

 

ABSTRACT

 

Various organochlorine pesticides currently used for various crops to protect them against insects. They are highly toxic, persistent and travel long distance through air and water causing pollution. Moreover, they are resistance to photolytic, chemical and biological degradation. Due to the uncontrolled and extensive use of pesticides creates the need to degrade them and restore the habitats. In the present study the biodegradation of organochlorine pesticides like Endosulfan (6,7,8,9,10,10-hexachloro-1,5,5a,6,9,9 a-hexahydro-6,M9-methano-2,4,3-benzo-dioxathiepine-3-oxide) and organophosphatepesticide methyl parathion (O,O-dimethyl-O-4-nitrophenylphos-phorothioate) have been studied using marine bacterium Nocardiopsis species isolated from the coastal area of the Gujarat, India. Bacterium was identified by 16srRNA gene sequencing. Isolate was found to degrade methyl parathion in liquid medium and three metabolites wereanalyzed on thin layer chromatography one of them was found p-nitrophenol. Degradation of endosulfan within 10 day in liquid culture was produced two distinct metabolites. Bioprospectives study suggest that isolate could produce the multiple commercial valuable enzymes e.g. Amylase, Protease, Chitinase, Cellulase, Pectinase. Moreover isolate was also reported to inhibited the growth of Bacillus subtilis and Fusarium species, indicates secretion of antibacterial and antifungal substance. Isolate suggest the great potential in removal of pesticide from soil and water bodies.

 

Keywords : Nocardiopsis species, Organophosphate, Organochlorine, Bioprospectives, Biodegradation

 

 

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