Volume No. 7 Issue No.: 2A Page No.: 936-945 Oct. - Dec. 2012




Kaur Rajdeep* and Pramanik Krishna

Department of Biotechnology and Medical Engineering, National Institute of Technology, Rourkela, Orissa (INDIA)


Received on : September 10, 2012




Biodegradation of tiger prawn waste was carried out using Lactobacillus plantarum (NCIM 2912) and Lactobacillus casei (NCIM 9595). Lactobacillus plantarum was found to be more efficient in term of % degradation. Detail investigation on the influences of key parameters such as glucose concentration, temperature etc. on biodegradation was done to study the efficiency of Lactobacillus plantarum for demineralization of tiger prawn shell waste. The fermentation experiments have been carried out using 150, 200, 250 and 300 micron particle size of shell waste and 8% inoculation as the starter culture upto 5days. Various concentrations of (0, 2, 4 and 8%) of glucose were supplemented as initial source of carbon for the microorganism. On the 5th day of fermentation the pH for varying glucose level (0, 2, 4 and 8%) was found to be 7.3, 6.9, 6.1 and 5.1. The corresponding Total Titratable Acidity (TTA) was observed as 0.67, 1.12, 1.7 and 6.2%. Maximum demineralization of 80% was achieved with particle size of 150 micron, at 8% glucose, 8% inoculum and 30°C. Correlation between pH and % Demineralization gave a negative relationship (R2=0.573) and a positive relation between pH and % demineralization (R2=0.860).


Keywords : Tiger prawn shell waste, Biodegradation, Lactobacillus plantarum, Lactobac-illus casei, Lactic acid fermentation, Demineralization, Biological waste treatment