Volume No. 7 Issue No.: 2A Page No.: 923-936 Oct. - Dec. 2012

 

A STUDY OF NATURAL POPULATION OF Neurospora AND ISOLATION OF NOVEL MORPHOLOGICAL MUTANTS

 

Mukati A., Vyas A.1 and Vyas H.*2

1. School of Studies in Microbiology,Vikram University, Ujjain (INDIA)
2. Department of Botany, Goverment Kalidas Girls’ College, Ujjain (INDIA)

 

Received on : September 10, 2012

 

ABSTRACT

 

Neurospora is a tropical fungus which is found abundantly growing on burnt sugarcane, discarded corncobs and other burnt vegetation in India. We have studied the population of Neurospora at three different sites in Madhya Pradesh, India. These three sites were - Railway station, Ujjain, Bilavli Lake, Khandwa road, Indore and Sanver Road (Sanver road side, in front of Trimurti Restaurant). At these sites Neurospora is found growing on corncobs which are discarded after eating the roasted kernel in rainy season. The visual conidial samples of Neurospora and soil samples from these sites were collected and brought to the laboratory. From these samples 136 Neurospora cultures were isolated and purified. The mating types and species of representative of Neurospora cultures were determined. The study shows that the most common species is Neurospora intermedia but some cultures belonging to Neurospora crassa have been found in Sanver and Indore. The yellow ecotype of N. intermedia is predominantly present at all the three sites but, orange ecotype of N. intermedia is also found at Sanver and Indore. Both the mating types of Neurospora are equally prevalent at all the three sites. We have screened the isolated Neurospora cultures for morphological mutants. Ten cultures have been identified which show distinct morphological defects. The defects in the morphology have been characterized and are described in this paper.

 

Keywords : Neurospora, Neurospora intermedia, Natural population, Morphological mutants, Mating types, Yellow and orange ecotypes of Neurospora

 

 

BACK