Volume No. 7 Issue No.: 2A Page No.: 869-875 Oct. - Dec. 2012

 

EFFECTS OF AN ONCOSTATIC DRUG ETOPOSIDE (VP16 – 213) ON SPERM MORPHOLOGY AND SPERM COUNT

 

Sastry M. S.*1 and Chaudhari R.M.2

1. Department of Zoology, R.T.M. Nagpur University, Nagpur (INDIA)
2. P.S.G.V.P. Mandal’s, G.B. Patel Science College, Shahada (INDIA)

 

Received on : August 10, 2012

 

ABSTRACT

 

Etoposide (VP16-213), an oncostatic drug was administered intramuscularly to six adult male Indian palm squirrel, Funambulus pennant (Wroughton) during the active breeding period at the dose level of 10 and 30mg/KgBW/day respectively with the object of examining its toxicities on the testis and accessory organs for 30 days. Both the treatments resulted into abnormalities in the sperm morphology, sperm count and motility. The various head defects were extreme elongation, compression, lateral displacement of head, loss of acrosome, amorphous or deformed head, compressed head with across ome thrown into bulb, loss of DNA and hence vacuolation in the head, pin-head condition, nuclear swelling, stippling in chromatin, unexpected curvature of the head, pyriform head. The mid-piece defects were-swollen mid-piece, loss of mid-piece. The tail defects were-unusually long and double tails. The sperm count was also low as compared to vehicle treated control and resulted into oligospermia (P<0.001), however with the high dose the condition resulted was oligozoospermia along with an increased percentage of sloughed off testicular cells in various stages of apoptosis. Sperm motility was of rotational type. Thus the study of etoposide conclusively demonstrated that this drug belongs to the category of antigonadotrophic, antiandrogenic and anti-spermatogenic leading to infertility when used in the cancer patients.

 

Keywords : Etoposide, Squirrel, Oligospermia, Oligozoospermia, Anti-spermatogenic

 

 

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