Volume No. 7 Issue No.: 2A Page No.: 863-868 Oct. - Dec. 2012

 

STUDIES ON PSYCHROTROPHIC Kocuria SPECIES FROM MILK AND FERMENTED MILK PRODUCTS

 

Mane Neeta V.* and Gandhi M. B.

Department of Microbiology, Yashwantrao Chavan College of Science, Karad, Vidyanagar, Karad, Satara, Maharashtra (INDIA)

 

Received on : July 10, 2012

 

ABSTRACT

 

Psychrotrophs are the organisms capable of appreciable growth at 7°C irrespective of the optimum growth temperature. These organisms can grow at wide range of temperature. They produce heat resistant, exoenzymes such as protease, lipase etc. during cold storage and attack proteins and fats in milk and milk products and cause spoilage problems. For studying these psychrotrophs milk and fermented milk samples from different dairies of Kolhapur region in Maharashtra were collected. Two psychrotrophic bacterial isolates obtained were belonged to genus Kocuria . They were characterized by studying their morphological, cultural, physiological and biochemical characters as well as 16S rRNA sequencing and further identified as Kocuria rosea and Kocuria sp.Mali 344. Lipolytic activities of these were studied. Both the isolates showed production of lipase and may hydrolyzed milk fat. Survival at pasteurization was also studied.

 

Keywords : Psychrotrophs, Lipolytic, 16S rRNA sequencing, Biochemical characters, Pasteurization

 

 

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