Volume No. 7 Issue No.: 2 Page No.: 643-651 Oct. - Dec. 2012

 

GAS-CHROMATOGRAPHIC DETERMINATION OF IMIDACLOPRID IN WATER

 

Srivastava Ashutosh K., Srivastava M.K., Patel D.K., Mudiam M.K.R. and Srivastava L.P.*

Pesticide Toxicology Laboratory, Indian Institute of Toxicology Research, (Council of Scientific and Industrial Research, Government of India), Lucknow (INDIA)

 

Received on : July 10, 2012

 

ABSTRACT

 

A simple and sensitive method for the analysis of imidacloprid in water has been developed using Gas Chromatography (GC). imidacloprid converted into a volatile imidacloprid-urea by alkaline hydrolysis. Water spiked with eight different concentrations of imidacloprid was mixed with NaOH and heated to obtain imidacloprid-urea. The hydrolyzed product was extracted with dichloromethane and finally taken into 1 ml mixture of acetone and n-hexane (1:9 v/v). The separation of peaks was done on gas chromatography coupled to Electron Capture Detector (ECD) and Nitrogen Phosphorous Detector (NPD) and further it was confirmed by Gas Chromatography - Mass Spectrometry (GC-MS). The mean recovery of imidacloprid-urea in water was found to be 92% .The percent Relative Standard Deviation (%RSD) of imidacloprid was found to be below 5% for different concentration levels. The limit of detection of imidacloprid-urea was found to be 20 mg L-1 with a signal to noise ratio of 3:1 and limit of quantification was found to be 75 mg L-1 with a signal of noise ratio 10:1. The developed method is sensitive, quick and easy to perform and could be utilized for regular monitoring of imidacloprid residue in water.

 

Keywords : Imidacloprid, Imidacloprid-urea, GC-ECD-NPD, GC-MS, Water

 

 

BACK