Volume No. 7 Issue No.: 1A Page No.: 411-415 July-September 2012

 

ROLE OF 28-HOMOBRASSINOLIDE ON SOME ANTIOXIDATIVE ENZYMES OF TOMATO PLANT DURING PATHOGENESIS

 

Kaur Ravinderjit, Ohri Puja*1 and Bhardwaj Renu2

1. Department of Zoology, Guru Nanak Dev University, Amritsar (INDIA) 2. Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar (INDIA)

 

Received on : May 05,2012

 

ABSTRACT

 

Brassinosteroids (BRs) are a new class of plant hormones, of wide occurrence in the plants. Alongwith their growth promoting effects, they are also reported to confer resistance to plants against various abiotic/biotic stresses. In the present study, the role of 28-homobrassinolide (HBl) was studied on some antioxidative enzymes of host plant during nematode infection. The surface sterilized seeds of tomato (var. Pusa Ruby) were pre-treated with different concentrations (10-11M, 10-9 M and 10-7 M) of HBl for 4 hrs. The seeds were then transferred to 80 mm sterilized petri-plates, lined with Whatman Sheet No-1 and kept in BOD incubator at 24°C. After germination, the seedlings were inoculated with second stage juveniles (J2) of plant-parasitic nematode, Meloidogyne incognita (@ 5 J2/ seedling). For each set of experiment, treated and inoculated plants were compared with untreated, uninoculated plants (control I) as well as untreated, inoculated plants i.e. control II. The specific activities of catalase, ascorbate peroxidase and guaiacol peroxidase were estimated seven days after inoculation (7DAI) in both roots and shoots. Results revealed that HBl treatment significantly increased the activities of all three enzymes, suggesting the role of these plant steroids in enhancing the enzymatic levels, which have helped the host plant to better survive during pathogenesis.

 

Keywords : 28-homobrassinolide, Catalase, Ascorbate peroxidase, Guaiacol peroxidase,

 

 

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