Volume No. 7 Issue No.: 1A Page No.: 405-410 July-September 2012

 

MUTAGENICITY OF ENDOSULFAN BY Salmonella typhimurium REVERSE MUTATION ASSAY

 

Mehta S.A.*1, Bhavsar B.S. and Nagane R.M2

1. C.G. Bhakta Institute of Biotechnology, Bardoli-Mahuva Road, Surat (INDIA) 2. Jai Research Foundation, Valvada , Valsad, Gujarat (INDIA)

 

Received on : May 05,2012

 

ABSTRACT

 

Endosulfan, an insecticide, is an endocrine disrupter and highly toxic. It is acutely neurotoxic to insects and mammals including humans. Objective of present study was to examine mutagenicity of endosulfan and evaluate non mutagenic concentration of it. Ames Salmonella mutagenicity assay was used as it specifically detects wide range of chemicals that can be mutagenic. Endosulfan at different concentrations was checked for its ability to induce reverse mutation at histidine locus in three strains of Salmonella typhimurium (TA98, TA100 and TA1537) in presence and in absence of metabolic activating system (S9) containing mammalian microsomal enzymes. Based on cytotoxicity test, 0.3125 and 0.15625 µof endosulfan/plate was selected as the highest concentration to be tested for mutagenicity test in the absence and in the presence of metabolic activation, respectively for all the tester strains. Endosulfan up to concentration of 0.3125 µ/plate in the absence and 0.15625 µ/plate in presence of metabolic activation was found to be non mutagenic to all three strains. There is no significant increase in number of revertant colonies accomplished that endosulfan with these concentration can be considered as non mutagenic.

 

Keywords : Endosulfan, Auxotrophic, Salmonella typhimurium, Mutagenicity, Ames test

 

 

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