Volume No. 7 Issue No.: 1A Page No.: 399-404 July-September 2012

 

SYNTHETIC SEEDS A VIABLE APPROACH FOR CONSERVATION AND PROPAGATION OF PHYTOREMEDIANT HERB : Bacopa monnieri (L.) WETTST

 

Sharma Munish, Raina Himani, Verma Vijeshwar, Mallubhotla Sharada*1 and Ahuja Ashok2

1. Tissue Culture Laboratory, School of Biotechnology, Shri Mata Vaishno Devi University, Kakryal, Katra, J & K (INDIA) 2. Plant Tissue Culture Unit, Indian Institute of Integrative Medicine (CSIR), Jammu, J & K (INDIA)

 

Received on : April 05, 2011

 

ABSTRACT

 

Bacopa monnieri (L.) Wettst. an emergent macrophyte, fast proliferating wetland species has attracted considerable attention in recent years owing to its capability of absorbing toxic heavy metals from the aquatic environment. As such plant species having dual importance need to be conserved and propagated on priority basis. A protocol was developed for regeneration from encapsulated nodal segments collected from in vitro propagated shoots of Bacopa monnieri. The best gel composition was achieved using 2.5% sodium alginate and 100 mM CaCl2.2H2O. The maximum percentage response (86.67) for conservation of encapsulated nodal segments into plantlets was achieved on Murashige and Skoog’s medium supplemented with 1.0 mg-1 BAP after 6-8 weeks. Well developed regenerated plantlets were hardened, acclimatized and established under net house conditions, where they grew well without any detectable variation. The regeneration ability of encapsulated nodal segments was affected by the concentration of sodium alginate and calcium chloride.

 

Keywords : Brahmi, Hyperaccumulation, Synseed, Micropropagation, Sodium alginate beads, Plantlet formation, Acclimatization

 

 

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