Volume No. 7 Issue No.: 1A Page No.: 338-345 July-September 2012

 

BIOSYNTHESIS OF SILVER NANOPARTICLES AND ITS ANTIFUNGAL ACTIVITIES

 

Tile Vaishali Arjun* and Bholay A. D.

Department of Microbiology, K.T.H.M College, Pune University, Nashik (INDIA)

 

Received on : May 25,2012

 

ABSTRACT

 

The development of a reliable process for the synthesis of silver nanoparticles is an important aspect of nanotechnology today. A narrow and controlled size preparation seems difficult as it depends on the concentration of reacting chemicals and controlled reaction environment. Chemical method for synthesis of silver nanoparticles uses toxic chemicals, which raises great concern for environmental reasons. It is known that microorganisms such as bacteria, yeast and fungi play an important role in the remediation of toxic metals through reduction of the metal ions. Only recently this approach is considered interesting as nanofactories. In this study, the production of silver nanoparticles by Escherichia coli and Bacillus subtilis was investigated, both intracellularly and extracellularly which were characterized by Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM) and UV-visible spectrophotometery. The silver nanoparticles showed potent activity against clinical isolates of Candida albicans, Tricophyton rubrum and Aspergillus fumigatous.

 

Keywords : Nanoparticles, Escherichia coli, Bacillus subtilis, Transmission Electron Microscope (TEM), Scanning Electron Microscope (SEM)

 

 

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