Volume No. 7 Issue No.: 1A Page No.: 312-320 July-September 2012

 

COMPARATIVE STUDY OF ANTIMICROBIAL ACTIVITY AND PHYTOCHEMICAL SCREENING OF AQUEOUS AND ALCOHOLIC LEAF EXTRACT OF Ocimum sanctum ON E.coli (FAECAL INDICATOR OF WATER POLLUTION)

 

Sadul Rama R.*1, Gidde Milind R.2 and Bipinraj N. K.3

1. Bharati Vidyapeeth Institute of Environment Education and Research, Pune (INDIA) 2. Department of Civil Engineering, B.V.D.U. College of Engineering, Pune (INDIA) 3. B.V.D.U Rajiv Gandhi Institute of I.T. and Biotechnology, Pune (INDIA)

 

Received on : May 05,2012

 

ABSTRACT

 

In the process of developing a plant based substitute for economical and safe method for water disinfection against conventional chemical constituents, the alcoholic and aqueous leaf extracts of Ocimum sanctum (Tulsi) were screened for evaluation of inhibition efficiency and the presence of phytochemicals. Ocimum sanctum is effective against E. coli and showed increase in antimicrobial activity with increase in dose and contact time . The antimicrobial activity of alcoholic leaf extract was more as compared to the aqueous leaf extract. The MPN test was performed as well, to compare the efficiency of alcoholic and aqueous leaf extract on waste water. A qualitative phytochemical analysis was performed on both the extracts for the presence of alkaloids, glycosides, terpenoids, steroids, flavonoids, tannins and reducing sugar. The alcoholic leaf extract contained alkaloids, steroids and tannins while the aqueous leaf extract contained only alkaloids and steroids which are active antimicrobial components in plants.

 

Keywords : Ocimum sanctum, Alcoholic leaf extract, Aqueous leaf extract, Antimicrobial, Water purification, Phytochemical analysis

 

 

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