Volume No. 7 Issue No.: 1A Page No.: 285-293 July-September 2012

 

ECONOMIC PRODUCTION OF MOLD XYLANASE BY LIGNOCELLULOSIC WASTE FERMENTATION FOR COMMERCIAL APPLICATIONS

 

Timande Sanjay P.

Dhote Bandhu Science College, Gondia, Maharashtra (INDIA)

 

Received on : May 25,2012

 

ABSTRACT

 

The production of xylanase using the fungus Aspergillus species S6 isolated from agriculture soil under submerged cultivation was investigated. Growth conditions including incubation time, temperature, inoculum size, initial pH, carbon and nitrogen sources that affect xylanase production were optimized sequentially using the classical change-one-factor-at-a-time method. Optimal xylanase production was obtained in liquid xylan medium at pH 5.5, inoculum size 6% v/v and temperature 300C during 7 days. Media supplementation with xylan as a carbon source (1% w/v) and nitrogen supplementation with ammonium nitrate (0.4% w/v) produced maximum xylanase. Enzyme yield was studied using a medium with different agricultural wastes as inducers. Wheat bran was the best inducer of xylanase activity. Influence of agitation of fermentation medium during incubation was studied. The optimum pH, temperature and substrate concentration for optimum enzyme activity were pH 6.0, 50°C, and 8 mg/ml, respectively. The Lineweaver-Burk plot for xylanolytic reaction revealed that the Vmax of the reaction was 0.714 mg/min and Michaelis–Menten constant (Km) was found to be 4.54 mg/ml. The xylanase produced was as an acid–active and displayed suitable thermo–stability feature.

 

Keywords : Aspergillus species S6, Xylanase, Agricultural wastes, Submerged fermentation, Agitation

 

 

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