Volume No. 7 Issue No.: 1 Page No.: 51-55 July-September, 2012


DETECTION OF Enterohaemorrhagic Escherichia coli (E. coli O157:H7) AND ITS DRUG RESISTANCE PATTERN


Naik Jigna*1 and Desai Pratibha2

1. Arts, Science and Commerce Collage, Kholwad, Surat, Gujarat (India)
2. Shree Ramakrishna Institute of Computer Education and Applied Science, Surat, Gujarat (India)


Received on : June 05,2012




Enterohaemorrhagic Escherichia coli (EHEC), is an emerging pathogen causing haemorrhagic colitis and Haemolytic Uraemic Syndrome (HUS). The most common EHEC is E.coli 0157:H7. Human may acquire EHEC infection primarily from consumption of contaminated food and drinking water or direct contact with feces of infected person. The treatment of this pathogenic E. coli infection is increasingly becoming difficult because of the multidrug resistance exhibited by the organism. In India, few research reports are suggesting that this enteropathogen may pose a serious public health problem. In present study an attempt was made to isolate EHEC from diarrheagenic stool sample of patients in Surat (India) and determination of their antimicrobial resistance pattern. Isolation and identification of E. coli was carried out from stool samples of patients with intestinal infection by standard procedure. E. coli isolates were inoculated for the identification of EHEC (E. coli O157:H7). The isolates were then further processed for the determination of antibiogram. 13.91 % isolates from 115 E.coli isolates were identified as EHEC. The least resistance was detected against Gentamicin (25%). Very high resistance was detected against Ampicillin(100%), Tetracycline(93.75%), Cefuroxime and Co-Trimoxazole (75%), Ceftriaxone, Chloramphenicol and Ciprofloxacin (68.75%). Our study findings indicate high frequency of EHEC in diarrheagenic stool sample. Multiple drug resistance was observed. Hence, routine screening of diarrheagenic stool sample for EHEC and their resistance pattern may be useful. However, further characterization of these isolates from large population of diarrheagenic individual and healthy control is necessary to know their role as emerging pathogens.


Keywords : Enterohaemorrhagic Escherichia coli (EHEC), E. coli O157:H7, Drug resistance, Pathogen, Haemolytic uraemic Syndrome (Hus)