Volume No. 6 Issue No.: 4 Page No.: 1066-1071 April-June 2012

 

BIODEGRADATION OF RESORCINOL BY

Pseudomonas

species ISOLATED FROM INDUSTRIALLY CONTAMINATED SOIL SAMPLE

 

Parmar P.*1, Vasavada G . and Desai P.2

1. C.G. Bhakta Institute of Biotechnology, Bardoli-Mahuva Road, Bardoli, Surat (India)
2. Biosciences Department, Veer Narmad South Gujarat University, Surat (India)

 

Received on : March 10, 2012

 

ABSTRACT

 

Phenolic compounds like phenol, resorcinol are widely used in chemical industries, in production of synthetic resins, plastics, polymers, dyes, pharmaceutical industries, as disinfectant waste, oil waste etc. Number of industries located an area of G.I.D.C in Surat, Ankleswar, Vapi which generates effluent containing phenolic compounds. Chemical and Physical treatments are used to remove it but having their own cost and not ecofriendly while biodegradation is an alternative method of it. Thus, attempt was made to isolate indigenous microbes from the contaminated sites which can utilize resorcinol as sole source of carbon and nitrogen. Resorcinol utilizing microbe was successfully isolated from the soil sample contaminated with industrially effluent, collected from the Amla Khadi near Ankleswar, Gujarat. The bacterium was successfully grown in Bushnell-Haas medium embedded with resorcinol as a sole source of carbon and nitrogen. Biodegradation studies with different parameters like resorcinol concentration 100 to1500 ppm, pH 4.0, 7.0, 10, temperatures 27°, 37°, 47°C were carried out. Our isolate shows maximum degradation with pH 7 at 37°C up to 1000ppm concentration on shaking conditions. Significant results were not seen with other parameters. On the basis of morphological, cultural and biochemical characteristics it was tentatitivly identified as Pseudomonas species.

 

Keywords : Biodegradation, Resorcinol, Bushnell-Haas medium, Pseudomonas species, Phenolic compounds

 

 

BACK