Volume No. 6 Issue No.: 4 Page No.: 1040-1043 April-June 2012

 

Genotoxicity assessment using micronucleus assays in Sperata seenghala at in-situ level from Lower Lake and Shahpura Lake, Bhopal,INDIA

 

R.K. Garg*, Nitin Batav and Rajesh Sharma

Centre of Excellence in Biotechnology, M.P. Council of Science and Technology (MPCST) Vigyan Bhawan, Nehru Nagar, Bhopal, Madhya Pradesh, India

 

Received on : March 05,2012

 

ABSTRACT

 

The present study was an attempt to explore the genotoxicity assessment in freshwater catfish, Sperata seenghala by micronucleus assay collected from Lower Lake and Shahpura Lake of Bhopal, India. The frequency of micronuclei was significantly higher in Shahpura Lake in comparison of Lower Lake. Total nuclei observed in Lower Lake were 44.00±0.811 and 56.00±0.944 in Shahpura Lake of which, the frequency of the micronucleus as 12.00±0.912 and nuclear abnormalities as 29.00±0.816 in Lower Lake, whereas, 16.00±1.414 and 30.75±0.577 were in Shahpura Lake. The results show that the assay can be employed for the evaluation and the assessment of water pollution and aquatic mutagens because Sperata seenghala fish is sensitive for bio-monitoring of genotoxicity as well as being an abundant species, easily may be kept with a wide distribution along the Central India.

 

Keywords : Mystus (Sperata) seenghala, Water Pollution, Genotoxicity, Micronucleus Assays (MN), Nuclear Aberrations (NA)

 

 

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