Volume No. 6 Issue No.: 4 Page No.: 1012-1018 April-June 2012


Use of response surface methodology to evaluate the extraction of Protein from Shrimp Waste by Aqueous Two-Phase system (Polyethylene glycol and ammonium citrate)


D. Ramyadevi, A. Subathira and S. Saravanan*

Department of Chemical Engineering, National Institute of Technology, Tiruchirappalli (India)


Received on : March 05,2012




Shrimp waste, which is produced in large quantities in the Indian seafood processing industries, is one of the important sources of protein. Studies were carried out to assess the extractability of shrimp waste protein in different aqueous two phase system and to optimize the extraction conditions for maximum yield. Four factors like PEG’s molecular weight (A), pH (B), Addition of sodium chloride (C), and Tie Line Length (D) that influence the protein partitioning were analyzed using response surface methodology. The optimum conditions were determined as PEG 4000, pH8, 1M Addition of sodium chloride, and 36 Tie Line Length. The optimal condition gave a partition coefficient for protein partitioning of 2.96 with a yield of 74.5%.


Keywords : Protein partitioning, PEG- Ammonium citrate aqueous two-phase systems, Response surface methodology