Volume No. 6 Issue No.: 3A Page No.: 721-726 Jan-Mar 12

 

BIOTECHNOLOGICAL UTILIZATION OF DAIRY WASTE TO SOLVE ENVIRONMENTAL PROBLEM

 

Swati Soni* and Sanjay Timande

P.G. Department of Microbiology, Dhote Bandhu Science College, Gondia, Maharashtra (INDIA)

 

Received on : September 10, 2011

 

ABSTRACT

 

To find an alternative mold source, nine molds were cultured in lactase production medium at room temperature in static condition for 7 days. Experimental results showed that Aspergillus species (L9) has maximum lactase activity at temperature 30° C after 5 days incubation. The optimized pH, size of inoculum and age of inoculum for maximum lactase production were 5.0, 6% v/v and 7 days. Lactase yield was determined by inoculating deproteinized cheese whey by hyperproducer under optimized conditions of fermentation.The best conditions for optimum activity of crude enzyme were determined. Lactase activity was maximum at pH 6.0 and temperature 45° C. The enzyme was stable upto 10 minutes at pH 6.0 and temperature 45°C. It was concluded that Aspergillus species (L9) could be used as an alternative for production of lactase on industrial scale utilizing deproteinized cheese whey. This strain can be used for removal of whey pollutants, SCP and ethanol production and treatment of lactose in dairy food.

 

Keywords : Lactase, Aspergillus, Deproteinized whey, Lactose intolerance Mold

 

 

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