Volume No. 6 Issue No.: 3A Page No.: 695-701 Jan-Mar 12

 

COMPARISON OF IMMOBILIZED WHOLE RESTING CELLS IN DIFFERENT MATRICES VIS-A-VIS FREE CELLS OF Bacillus megaterium FOR ACYLTRANSFERASE ACTIVITY

 

Monika Sogani*, Nupur Mathur, Pratibha Sharma1 and Pradeep Bhatnagar2

1. Department of Zoology, University of Rajasthan, Jaipur, (INDIA)
2. IIS University, Mansarovar, Jaipur, (INDIA)

 

Received on : November 20, 2011

 

ABSTRACT

 

The bacterial isolate Bacillus megaterium was isolated from the soils of contaminated site of pharmaceutical industry, Sitapura, Jaipur and identified as an amide hydrolysing bacterium. Amidase, an amide-hydrolyzing enzyme also shows acyltransferase activity. The use of acyltransferase or bacteria with microbial enzymes with acyltransferase activity may be used to convert amides to Hydroxamic acids. The whole resting cells of Bacillus megaterium were prepared and assayed for acyltransferase activity spectrophotometrically. Known amount of whole resting cells of Bacillus megaterium were immobilized in the gel beads of sodium alginate, agar, polyacrylamide and PVA-alginate. These immobilized beads were used repeatedly as biocatalysts in 10 reactions to test their reusability potential. The whole cells immobilized into four different matrices were tested for the thermal stability and reusability potential in comparison to free cells. This study proved useful in understanding the technique of immobilization of acyltransferase enzyme, its operational stability and its importance in the synthesis of hydroxamic acid.

 

Keywords : Immobilized cells, Acetamide, Hydroxamic acid, Acyltransferase, Alginate, Agar polyacrylamide, PVA-alginate

 

 

BACK