Volume No. 6 Issue No.: 3A Page No.: 688-694 Jan-Mar 12

 

CHARACTERIZATION AND OPTIMIZATION OF SIDEROPHORE PRODUCTION FROM Pseudomonas fluorescens STRAIN ISOLATED FROM SUGARCANE RHIZOSPHERE

 

Aparna J. Tailor* and Bhavesh H. Joshi

1. C. G. Bhakta Institute of Biotechnology,Gopal Vidyanagar, Surat, Gujarat (INDIA)
2. Department of Food Quality Assurance, College of Food Processing Technology and Bioenergy, Anand Agricultural University Anand, Gujarat (INDIA)

 

Received on : November 25, 2011

 

ABSTRACT

 

Extensive screening for the siderophore producing bacteria from the sugarcane rhizosphere was carried out. Seven isolates were found to produce more than 85 % siderophore units. Amongst them S-11 was found be the most efficient siderophore producer (96 % SU). S-11 was further characterized and identified as Pseudomonas fluorescens. Physico-chemical parameters were evaluated for optimum for production of siderophores by Pseudomonas fluorescens strain. It was found to produce maximum siderophore at pH 7 and 29°C. Tyrosine as carbon source was found to stimulate bacterial growth as well as siderophore production. Maximum siderophore yield was obtained with ammonium sulphate and urea as independent nitrogen source. Iron concentration up to 20 μ M was found to optimum for siderophore production. Shake flask studies revealed that the siderophore pro- duction starts after 6 h of growth and reached to maximum productivity of 96 % SU after 24 h. The present study reveals Pseudomonas fluorescens strain as a promiscus candidate for crop improvement and protection due to its PGPR activities.

 

Keywords : Rhizospheric, Siderophores, % SU, PGPR. Carbon, Nitrogen

 

 

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