Volume No. 6 Issue No.: 2 Page No.: 281-285 Oct-Dec 2011

 

HISTOPATHOLOGY OF Larve carassius GIBELIO BIOMARKER OF POLLUTION WITH CYANOBACTERIA OF SHKODRA LAKE WATER, Albania

 

Medja N.* and Golemi S.

Department of Biology, University of Shkodra (ALBANIA)

 

Received on : September 15, 2011

 

ABSTRACT

 

The aim of this study was to find out how crude extract of cyanobacteria can influence larval development of carassius on the basis of embryo-larval toxicity test and histological changes of liver of larvae carassius exposed 30 days to the crude extract of cyanobacteria with the cumulative concentration 9.0 μg l–1 (medium concentration of the extract) and 0.9 μg l–1 (low concentration of the extract) of microcystins LR, RR and YR. The experiments were finished after 30 days. Evaluation of the tests was based on the OECD guideline for testing chemicals, direction 210 from 1992. Liver sections were stained with haematoxylin-eosin and using light microscopy. The extract with medium concentration caused an increase in malformed and dead larvae. The extract with low concentration caused an increase in dead larvae. Vacuolar dystrophy of hepatocytes accompanied by damage of nuclei (pyknosis,) were found in the group exposed to the low concentration of the extract. Focal necroses and dystrophic changes of hepatocytes with vacuolization and nuclei damage (pyknosis, karyolysis,) were found in the group exposed to the medium concentration of the extract. The degree of damage depended on the concentration of the extract.

 

Keywords : Hepatocyte, Cyanotoxins, Malformations, Fish, Embryo-larval toxicity test

 

 

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