Volume No. 5 Issue No.: 4 Page No.: 892-897 April-June 2011




Bhowal M.*, Cherian K. J. and Das L.

Department of Botany, Hislop College, Nagpur (INDIA)


Received on : February 25, 2011




Trifolium alexandrium L. commonly known as Berseem or Clover is an important leguminous winter fodder crop. It is appreciated as a forage crop due to its high protein content, soft leaves, tender stem, high leaf to stem ratio and also rapid growth. In comparison to other legumes and grasses, Clover provides forage with high nutritional value and quality. Biotechnology is a technique for the improvement of crop plants. The objective of the present study is to regenerate Trifolium alexandrium plants by tissue culture technique. A protocol for direct organogenesis was developed for this particular plant by inoculating the explants (shoot tip, cotyledonary node without cotyledons, cotyledon and hypocotyledon) on different media like MS and L2. Shoot tip was found to be the best responsive explants for direct organogenesis. Subsequently, it was found that 75% of explants produced shoots on L2 media supplemented with BAP (1mg/L) and IAA (0.1mg/L). The shoots were produced after 5 days of inoculation. Direct organogenesis from the shoot tip gives a pure line which can be utilized in genetic improvement of this crop plant. The shoot tip propagated plants are the stock for further production of large number of plant whenever necessary. This will cut down the conventional method of growing the crop by sowing and its subsequent growth. These plants grown by tissue culture technology can be preserved as germplasm.


Keywords : Berseem, Explants, Germplasm, L2 medium, Organogenesis