Volume No. 5 Issue No.: 2 Page No.: 413-420 October-December 201

 

ENVIRONMENTAL FACTORS AFFECT THE SURVIVAL AND EXPRESSION OF VIRULENT CYTOLETHAL DISTENDING TOXIN OF Campylobacter jejuni

 

Deepika Prasadi1 and K. N. Prasad*2

1. Allenhouse Institute of Technology, Kanpur (INDIA)
2. Department of Microbiology, Sanjay Gandhi postgraduate institute of Medical Sciences, Lucknow (INDIA).

 

Received on : July 02, 2010

 

ABSTRACT

 

Several environmental factors regulate the expression of virulence genes under certain conditions and the expression of bacterial virulence determinants. Cytolethal distending toxin (CDT) is an important virulence determinant secreted by Campylobacter jejuni (C. jejuni), a leading cause of gastrointestinal bacterial pathogen worldwide. Therefore, the aim of the study was to investigate the effect of selected environmental stress factors such as temperature, pH and proteolytic enzymes on cytotoxicity of culture supernatants containing CDT activity of 10 C. jejuni strains [5 CDT (-ve) and equal number of randomly selected CDT (+ve) C. jejuni strains] using Hep-2 and HeLa cells. Additionally, after stress exposure, adhesion, invasion and intracellular survival of C. jejuni were also studied.

 

Keywords : Campylobacter jejuni, Cytolethal distending toxin, Stress, Cytotoxicity, Epithelial cell line

 

 

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