Volume No. 4 Issue No.: 4 Page No.: 983-991 April-June 2010

 

ISOLATION AND CHARACTERIZATION OF LACTIC ACID BACTERIA FROM DAIRY EFFLUENTS

 

Barnali Ashe and Subhankar Paul*

Department of Biotechnology and Medical Engineering, National Institute of Technology, Rourkela, Orissa, (INDIA)

 

Received on : January 16, 2010

 

ABSTRACT

 

Lactic acid bacteria usually used as starter cultures in food technology are known to manufacture antimicrobial products having immense potential. Our study aims to isolate potential strain of lactic acid bacteria which has a potential for lactic acid production, to characterize it and to optimize culture conditions for the process. For this we have isolated a total of 38 strains of lactic acid bacteria (LAB) from dairy effluent samples that were collected from OMFED dairy plant of Rourkela, Orissa, (India). The isolated strains were identified on basis of their morphological, physiological and biochemical characteristics. The distribution of the isolates by genus was as follows: Staphylococcus, Lactobacillus, Brevebacterium, Streptococcus, Leuconostoc and Lactococcus. The colonies, which showed zone of clearance in starch agar plates, were maintained on MRS agar plate for further experiments. Quantification of Lactic acid conversion from starch into Lactic acid by the selected microbial isolates was carried out.

 

Keywords : Lactic acid bacteria (LAB), Isolation, Dairy effluents, MRS agar plate, Starch degradation, Bacterial strain

 

 

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