Volume No. 4 Issue No.: 4 Page No.: 947-957 April-June 2010

 

INFLUENCE OF MOISTURE CONTENT AND OXYGEN CONCENTRATION ON BIODEGRADATION OF PETROLEUM HYDROCARBONS

 

B.G. Mahendra*, B. Santhaveerana Goud and G.M. Srinidhi

Faculty of Engineering-Civil, UVCE,Bangalore University, Bangalore, (INDIA)

 

Received on : December 24, 2009

 

ABSTRACT

 

The biodegradability of petroleum hydrocarbons was evaluated using petroleum oily sludge from VRL logistics ltd situated at Kengeri, Bangalore (India). The soil rich in native microorganisms was collected from Bangalore University campus and the same is used to prepare simulated contaminated soil. The initial Total petroleum hydrocarbons (TPH) concentration in the simulated contaminated soil was 83,940 mg /kg of soil. Biodegradation was examined for three different conditions i.e. four bioreactors for studying variation of moisture, four bioreactors for studying variation of oxygen and one bioreactor as control Treatability studies on TPH contaminated soil was conducted for 12 weeks to evaluate TPH mass loss rates under the most favorable conditions, for which a set of nine bioreactors each with 15 kg of fresh soil, 3 kgs of oily sludge, 1.5 kgs of inoculated soil in the ratio of (10:2:1) were thoroughly mixed and maintained under laboratory conditions. The TPH, moisture content, pH, bacterial counts and oxygen were monitored regularly along with the nutrient concentration i.e.. C: N: P ratio maintained at 100:10:1 in all the bioreactors except the control reactor. From the study it is concluded that the optimal conditions for better degradation of TPH is found to be between 50% - 60% of moisture content with biodegradation rate of 0.0128 - 0.0174 day1 and TPH removal efficiency of 68.5 - 79.2% and oxygen concentration of 50 - 60 mg/kg/day, with biodegradation rate of 0.0120

 

Keywords : Biodegradation, Moisture, Oxygen, Hydrocarbons, Soil, Bioreactor

 

 

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