Volume No. 4 Issue No.: 3 Page No.: 668-677 Jan.-Mar 2010




M. H. Soliman*, H. Saad1 and N. Gadallah2

1. Chemistry Department, Faculty of Science, Helwan University, Helwan, (EGYPT)
2. National Organization of Drug Control and Research, Cairo, (EGYPT)


Received on : December 16,2009




Three accurate methods are developed for determination of sulpiride in pure form and in its dosage form: spectrophotometry using three new reagents 2,3-dichloro-5,6-dicyano-p-benzoquinone (DDQ) and 7,7,8,8- tetracyanoquinodimethane (TCNQ) as π-acceptors and iodine σ-acceptor via charge transfer (CT) reactions, TLC and HPLC. Three charge transfere (CT) reactions between DDQ, TCNQ and iodine have been spectrophotometrically studied. The optimum experimental conditions have been studied carefully. Beer’S law is obeyed over the concentration range of 10-90 μg ml-1 for DDQ, 15-100 μg ml-1 for TCNQ and 5-85 μg ml-1 for iodine reagent, respectively. The sandell sensitivity is found to be 0.071, 0.030 and 0.022 μg cm-2 for DDQ, TCNQ and iodine methods, respectively which indicates the high sensitivity of the three methods. Thin layer chromatographic method (TLC) was carried out using developing system of acetonitrile: water : glacial acetic acid (10:10:1 v/v/v) followed by densitometric measurement of the spots into at drugs at 290 nm). The limits of Beer’S law was 5-60μg spot-1 . HPLC was carried out using Luna CN 150x4.6 mm column. The precision and accuracy of all the methods were complexively compared with those obtained by British and American pharmacopoeias for the determination of sulpiride in raw materials and in pharmaceutical preparation .


Keywords : Spectrophotometry, Thin layer chromatography, High pressure liquid chromatography, 2,3-dichloro 5-6-dicyanoquinone (DDQ), 7,7,8,8-tetracyanoquino-dimethane (TCNQ), Iodine, Sulpiride, Pharmaceutical preparations