Volume No. 1 Issue No.: 1 Page No.: 16-17 July-September 2006

 

PYRIDINE BASED PYRAZOLINES : SYNTHESIS AND ITS IMPACT ON CYTOTOXIC ENVIRONMENT IN SPLEENOCYTES

 

Alka Pradhan* and Meghna Mehndiratta

Motilal Vigyan Mahavidyalaya, Bhopal (INDIA)

 

Received on : April 29, 2006

 

ABSTRACT

 

Attempts to establish relationship between chemical structure and biological activities of therapeutic agents, have always been interesting in medicinal chemistry. Pyrazolines, an interesting heterocycles, are studied extensively because of their ready accessibility and diverse chemical activity and broad spectrum of biological and pharmacological properties. This Research, mainly deals with the synthesis and its impact on chromosomes of spleenocytes i.e., an internal body environment with respect to pyrazolines. Here, some new pyridine based pyrazolines are synthensised. Substituted series of 2-(2’-amino pyridine)-carboxamido were achieved gradually by reacting varied substituted anilines alongwith di-ethyl malonate, followed by the condensation with 2-amino pyridine for about a hour. Former, when condensed in presence of benzaldehyde and conc. H2SO4 yielded the conclusive intermediate of 2-(2’-amino pyridine)-carboxamido-3-phenyl-ethene (N-subst. anilide), which when stirred with hydrazine hydrate in an ethanolic medium furnished shiny, needle shaped crystals of 2-(2’-amino pyridine) carboxamido-3-(N-subst. anilido)-5-phenyl pyrazolines. Total eight compounds had been screened for determining their anti-microbial spectrum of effectiveness against pathogens like S. aureus, E. Coli and P. aeruginosa; C. albicans, A. fumigatus and A. Flavus. Pyrazolines exhibited an excellent activity against S. aureus (MIC 125

 

Keywords : Animal behaviour, Apoptosis, Cytotoxicity, m (Albino mice), MN (micro nuclei), Spleenocytes

 

 

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