Volume No. 4 Issue No.: 1 Page No.: 49-54 July-Sept 2009

 

PRODUCTION AND EXTRACTION OF ?-GALACTOSIDASE FROM MARINE DERIVED FUNGUS A. flavus

 

Pavani Anumukonda* and Prabhakar Tadimalla

Department of Pharmaceutical Sciences, Andhra University, Visakhapatnam (INDIA)

 

Received on : May 05, 2009

 

ABSTRACT

 

?-galactosidase (?-D-galactoside galactohydrolase, E.C. 3.2.1.23), most commonly known as lactase, is one of the most important enzymes used in food processing, which catalyses the hydrolysis of lactose to its constituent monosaccharides, glucose and galactose. The enzyme has been isolated and purified from a wide range of microorganisms. The most commonly used ?-galactosidases are derived from bacteria, yeast and fungal sources. The major difference between yeast and fungal ?-galactosidase is optimum pH and temperature for lactose hydrolysis. The application of ?-galactosidase for lactose hydrolysis in milk and whey offers nutritional, and environmental applications to human life.

A. flavus was screened from marine fungus and grown on lactose for the production of ?-galactosidase. The preparation contained biomass of a selected fungal isolate of the Aspergillus flavus intracellular ?-galactosidase was made freely accessible to its substrate by permeabilization of the cell membrane with different chemical reagents. Among various chemical reagents ethanol and toluene were found to be the best permeabilizing agents for the extraction of ?-galactosidase.

 

Keywords : ?-galactosidase, Lactose hydrolysis, Marine derived fungus, Permeabilizing agents.

 

 

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