Volume No. 3 Issue No.: 4 Page No.: 1194-1203 April-June 2009

 

OPTIMIZATION OF PROCESS CONDITIONS USING RESPONSE SURFACE METHODOLOGY (RSM) FOR ETHANOL PRODUCTION FROM WASTE CASHEW APPLE JUICE BY Saccharomyces cerevisiae

 

M. Karuppaiya*, T. Viruthagiri and V. Vijayagopal

Bioprocess Engineering Research Laboratory, Department of Technology, Annamalai University, Annamalai Nagar (INDIA)

 

Received on : February 10, 2009

 

ABSTRACT

 

Bioethanol is an important renewable energy resource. It is blended with fuel have much higher compression ratio without Octane boosting additive. Ethanol has been known for a long time, being perhaps the oldest product obtained through traditional biotechnology. It is an attractive, sustainable energy source to fuel additives. Based on a four level central composite design (CCD) involving the variables substrate composition (X1), pH (X2), incubation temperature (X3) and fermentation time (X4), a response surface methodology (RSM) for the production of ethanol from cashew apple juice by Saccharomyces cerevisiae. The design contains a total of 31 experimental trials with the first 16 organized in a fractional factorial design and 25 to 31 involving the replications of the central points. Data obtained from RSM on ethanol production were subjected to the analysis of variance (ANOVA) and analyzed using a second order polynomial equation resulted in the optimized process conditions of 62% (v/v) as substrate concentration, pH

 

Keywords : Ethanol, Central composite design (CCD), Response surface methodology (RSM), Cashew apple juice.

 

 

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