Volume No. 1 Issue No.: 2 Page No.: 174-177 October-December 200

 

MONITORING OF HEAVY METAL (MERCURY CHLORIDE) TOXICITY BY USING POLLEN AS INDICATORS - POLLEN OF VIGNA UNGUICULATA A CRITICAL REVIEW

 

S. A. Salgare* and Suwarna Gawde

Salgare Research Foundation Pvt. Ltd. Prathamesh Society, Shivaji Chowk, KARJAT - 410 201, (India)

 

Received on : July 10, 2006

 

ABSTRACT

 

Seeds of Vigna unguiculata (L.) Walp. var. Pusa barsati (cowpea) were sown in white-transparent polythene bags (35 x 25 cm) containing garden soil. Each bag contains 20 seeds. These bags were watered on every alternate day for the first 7 days. After 7 days each bag was separately treated with the different concentrations (0.001, 0.01, 0.1, 1, 10, 100 mg/ml) of mercury chloride with the quantity of 500 ml on every alternate days till the end of the life-cycle of the crop. Excess seedlings were removed after 15 days of sowing leaving the identical and healthy 5 seedlings in each bag. Cent percent mortality of V. unguiculata was caused by 100 mg/ml of the heavy metal after one week of treatment. After 3 weeks of treatment of 10 mg/ml mercury chloride prevented cent percent germination of pollen of all the 4 series, while even the treatment of 1 mg/ml heavy metal suppressed the germination of pollen of F-48 and F-72 series. The treatment of 0.001, 0.01, 0.1 mg/ml stimulated the germination of F series. All the different concentrations of the heavy metal tried inhibited the germination of pollen of F-24, F-48, F-72 series of V. unguiculata. The treatment of all the concentrations of the heavy metal inhibited the pollen tube growth of all the 4 series of successive flowers. All these observations were made after 3 weeks of uniform flowering in all the sets.

 

Keywords : Heavy metals, Crop Physiology, Toxicology, Environmental Sciences.

 

 

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