Volume No. 3 Issue No.: 3 Page No.: 677-684 Jan-Mar 2009

 

OPTIMIZATION OF HEAVY METAL BIOSORPTION USING ATTENUATED CULTURES OF Bacillus subtilis AND Pseudomonas aeruginosa

 

K. Tarangini1 and Gyana R. Satpathy*2

1. Department of Chemical engineering, N.I.T, Rourkela (INDIA)
2. Department of Biotechnology and Medical Engineering, N.I.T., Rourkela (INDIA)

 

Received on : November 20, 2008

 

ABSTRACT

 

Biosorption can be a part of to solve the water pollution problem caused due to toxic heavy metal contamination resulting from humans technological activities. Several gram positive and gram negative bacteria have the ability to adsorb the heavy metals and helps in effective cleaning of contaminated water and it has been documented that attenuated bacterial biomass have greater biosorption capability than viable cells. In the present study, biosorption of Chromium (Cr), using mixed culture of attenuated gram positive and gram negative, bacteria like Bacillus subtilis and Pseudomonas aeruginosa and parameters affecting the biosorption of heavy metals, such as time, pH, biomass concentration and initial metal concentration, have been investigated. The comparative experiments have been carried out using individual and mixed bacterial culture and the biosorption parameters were optimized. Analytical results of the study revealed that 85% of biosorption of chromium was observed for mixed cultures of Bacillus subtilis and Pseudomonas aeruginosa and 60% and 50% biosorption for individual cultures respectively in 30 min which was optimum. And also other parameters for Cr biosorption were established for mixed biomass cultures and found to be 32oC, 3 pH, 300 rpm agitation and 1gm/l of biomass concentration.

 

Keywords : Biosorption, Heavy metals, Attenuated cells, Optimization

 

 

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