Volume No. 3 Issue No.: 3 Page No.: 601-606 Jan-Mar 2009

 

EVALUATION OF IMPORTANT TRITERPENES IN FIELD AND IN VITRO PLANTS OF Syzygium Cordatum

 

Nisha Singh*, Sharda M Potukuchi1 and Francis Oluwole Shode2

1. School of Biological and Conservation Sciences, University of KwaZulu-Natal (SOUTH AFRICA)
2. School of Chemistry, University of KwaZulu-Natal, Westville Campus, Private Bag X54001, Durban (SOUTH AFRICA)

 

Received on : November 08, 2008

 

ABSTRACT

 

Syzygium cordatum Hochst.ex C.Krauss (Myrtaceae), a recalcitrant tree species, commonly known as umdoni or water berry is known for its many uses. Extracts of the plant are taken as remedies for various ailments including tuberculosis. We detected betulinic acid, an important triterpene in leaf extracts. Betulinic acid is reported to selectively induce apoptosis in human melanoma cells only in anti-cancer clinical trials. Furthermore, its inhibitory activity against HIV-1 replication has also been reported. Our study focuses on the mass propagation of whole plants or selected organs, tissues or cells of this species for the development of in vitro protocols for the bulking of betulinic acid, mainly by using bioreactor technology. Seed germination on MS medium was 100% and occurred within 2-3 days. Following transfer to larger vessels, healthy rooted green plants developed within 1-2 weeks. Protocols for suspension and root cultures are being developed. Field and in vitro leaf material separately oven-dried and grounded into fine powders were extracted sequentially in hexane, dichloromethane and ethyl acetate and methanol. TLC analysis indicated that the ethyl acetate extracts contained better quantities of betulinic acid. These extracts were further eluted by column chromatography. The BA fractions are being analysed by NMR for verification.

 

Keywords : Betulinic acid, Micropropagation, Oleanolic acid, Recalcitrant, Triterpene, Tissue culture, Umdoni

 

 

BACK