Volume No. 11 Issue No.: 4 Page No.: 725-739 April-June 2017

 

OPTIMISATION OF GROWTH FACTORS FOR EFFECTIVE USE OF PHOSPHATE SOLUBILIZING BACTERIAL STRAINS AND USE AS BIOINOCUL- ANTS FOR GROWTH OF GROUND -NUT (Arachishypogaea) PLANT

 

Anbalagan Priyanka and Krishnaswamy Veenagayathri*

Department of Biotechnology, Stella Maris College,Cathedral road, Chennai, Tamil Nadu, (INDIA)

 

Received on : December 03, 2016

 

ABSTRACT

 

Phosphorus (P) is one of the essential macronutrients for plant growth and reproduction. Plants acquire Phosphorus from the soil solution as phosphate anions. However, phosphate anions are extremely reactive and may be immobilized through precipitation with cations such as Ca2+, Mg2+, Fe3+ and Al3+, depending on the particular properties of the soil and as a result, the phosphate is highly insoluble and unavailable to plants. Phosphate-solubilizing bacteria (PSB) has been added as fertilizer to increase Phosphorus uptake and plant growth. It is usually observed that the solubilization of phosphate by the phosphate solubilizing bacteriawould drop thepH.Acidification was due to production of organic acids by the bacterial strains.Such plant growth-promoting bacteria has the ability of that produce enzymes such as Phosphatase and 1aminocyclopropane-1-carboxylate (ACC) deaminase to lower plant ethylene levels, that often results in various stresses, which shows the efficacious functioning of these bacteria. This study aims to determine the optimal growth factors of the isolated phosphate-solubilizing Bacteria for use as a Biofertilizer, specificallyto check effects of temperature, pH and different carbon, nitrogen sources and NaCl concentrations on phosphate solubilization. Phosphate solubilization was expressed maximum at pH 6, temperature 25 C,Dextrose as carbon source, Ammonium sulphate as Nitrogen source and (1.2%) NaClconcentrations for the isolated phosphate solubilizing bacterial strains. Further the Phosphate solubilizing bacteria was applied to enhance the root, shoot length and Phosphorus accumulation content of ground nut (Arachishypogaea) seedlings. Present study highlights the importance of these plants growth promoting bacterial strains and their uses for agriculture purposes as a biofertilizer.

 

Keywords : Phosphate solubilizing bacteria, Optimization, Phosphatase, ACC deaminase, Groundnut, Biofertilizer

 

 

BACK