Volume No. 11 Issue No.: 4 Page No.: 646 -659 April-June 2017

 

CHARACTERIZATION OF PLANT GROWTH PROMOTING BACTERIAL STRAINS FROM RARE EARTH MINERAL DEPOSITS OF COASTAL REGION OF BAY OF BENGAL, ODISHA, INDIA

 

Mohapatra Balaram*, Ray Pratima1, Dutta Himanshu Sekhar2, Maharana Jitendra3 and Verma Deepak Kumar4

1. Post Graduate, Department of Microbiology, Orissa University of Agriculture and Technology, Bhubaneswar, Odisha (INDIA)
2. Department of Biotechnology, Indian Institute of Technology, Kharagpur, West Bengal, (INDIA)
3. Department of Bioinformatics, Orissa University of Agriculture and Technology, Bhubaneswar, Odisha (INDIA)
4. Department of Agricultural and Food Engineering, Indian Institute of Technology, Kharagpur, West Bengal, (INDIA)

 

Received on : December 12, 2016

 

ABSTRACT

 

In a laboratory study, ten mesophilic, heterotrophic plant growth promoting bacterial strains were isolated from heavy mineral deposits of the coastal region of the Bayof Bengal, Odisha. All were characterized for PGP activities: production of ammonia, siderophore, lytic enzymes, indole acetic acid (IAA), phosphate-solubilizationand heavy metal resistance. Five showed IAA production ranged from 1.60-4.25 mgL-1. Enhanced production was noticed with addition of Ltryptophan; 2.10 to 19.25 mgL-1. Six were able to solubilize insoluble calcium phosphate by production of acid with change in medium pH, where IREB 9 found to be most potent as it showed 90% phosphate solubilizationand showed phylogenetic lineage with the members of the genus Bacillus sp.All strains produced ammonia which ranged from 10.0-281.0 mgL-1 with four showing abilities to produce siderophores. Eight strains showed amylase activity followed by cellulase, lipase, Chitinaseand DNase.The antibiotic test showed that almost all strains (except IREB 5 and IREB 7) were resistant against streptomycin,chloramphenicol, nalidixic acid and polymixin-B. All heavy metals (except Hg) tested were found to having high resistancevalue for all the strains. The indigenous bacterial strains found in this study showed many plant growth promoting traits andcould be helpful in improving plant cover and soil health in heavy mineral contaminated environment in future.

 

Keywords : Heavy mineral, Bacterial strains, Plant growth promotion, Bacillus sp, Contaminated environment

 

 

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