Volume No. 11 Issue No.: 1 Page No.: 151-155 July-September 2016

 

MYCORRHIZAL INOCULATION ENHENCES GROWTH AND SAPONIN ACCUMULATION IN MICROPRO - PAGATED Chlorophytum borivilianum

 

Patel A.*2 Shrivastava A.1 and Dwivedi S.2

1. Department of Botany and Biotechnology, Government Science College Rewa, Madhya Pradesh (INDIA)
2. Department of Environmental Biology and Biotechnology, Awadhesh Pratap Singh University, Rewa, Madhya Pradesh (INDIA)

 

Received on : April 09, 2016

 

ABSTRACT

 

Micropropagated plantlets of Chlorophytum borivilianum exhibit high mortality rate upon their transfer to soil as a result of transplantation shock caused by biotic and abiotic stresses and weak root system in the absence of beneficial microflora. Biotization of micropropagated plantlets with the vesicular-arbuscular mycorrhizal fungus Glomus fasciculatum improved formation of a well-developed root system that was converted into a mycorrhizal system. Introduction of the mycorrhizal fungus at the time plantlets were transferred from in vitro to ex vitro conditions improved shoot and root growth, saponin accumulation and helped plants to tolerate environmental stress at transplanting. After 270 days, saponin content and root growth in all the mycorrhizal inoculated plants was found to be greater than in non-mycorrhizal material. Thus, mycorrhizal inoculation improved survival and secondary metabolite accumulation by micropropagated C. borivilianum.

 

Keywords : Safed musli, Glomus faciculatum, Mycorrhiza, Saponin, C. borivilianum

 

 

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