Volume No. 11 Issue No.: 1 Page No.: 92-103 July-September 2016

 

POLYHYDROXYALKANOATE PRODUCING NOVEL Bacillus SP., SKM123 ISOLATED FROM POLLUTED POND WATER

 

Mahmood S. K. and Chaitanya K.*

Department of Botany, Osmania University, Hyderabad, Telangana State (INDIA)

 

Received on : March 10, 2016

 

ABSTRACT

 

A gram-stain-positive, rod-shaped, motile and halo tolerant bacterium, was isolated from the polluted pond water. Strain grew at 1545oC (optimum 30oC) and pH 5.09.0 (optimum pH 7.0) and in the presence of 015 % (w/v) NaCl optimum 1 % (w/v). The strain was catalase-positive and oxidasepositive. The cell wall phospholipids were analyzed. The major fatty acids of the strain were anteiso- C15:0 (37.81%) and iso-C (30.14%). The DNA G+C content was 45.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain is a member of the genus Bacillus and is most closely related to Bacillus sonorensis NBRC 101234T (AYTN01000016) (97.74%), Bacillus aerius 24KT (AJ831843) (97.72%), Bacillus licheniformis ATCC14580T (AE017333) (97.68%). The sequence of the 16S rDNA gene of strain was determined 1550bp and deposited in the Genbank sequence database under accession no. LM655322. The phenotypic, chemotaxonomic and genotypic properties clearly indicate that strain represents a novel Polyhydroxyalkanoate producing species of the genus Bacillus. The strain produced PHA in mineral medium consisting of glucose and nitrogenous substances. Optimum conditions for polymer production were determined, extracted and confirmed through Spectroscopic, GC-MAS, GPC and IR analysis. Strain SKM123= KCTC33684T/MCC-2826T.

 

Keywords : Bacillus sp., Polyhydroxyalkanoate, NaCl, Spectroscopic, IR analysis

 

 

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