Volume No. 11 Issue No.: 1 Page No.: 72-79 July-September 2016

 

SILK THIN FILM FOR CELL AND TISSUE GROWTH

 

Sampratha B.*1, Navale Prakash M., Thippa Reddy K. S. and Agrawal P.2

1. Department of Biotechnology, P.E.S. Institute of Technology, Bangalore (INDIA)
2. Department of Biotechnology, R. V. College of Engineering, Bangalore (INDIA)

 

Received on : April 10, 2016

 

ABSTRACT

 

Silk fibroin is an important component of Bombyx mori cocoon which has been predominantly exploited as the textile material. In the recent times, it is widely used in the biomedical field due to its material and surface properties. The distinguishable biological nature of silk fibroin including good cytocompatibility, biocompatibility, no immunogenic reactions and oxygen permeability makes it a suitable biomaterial. It can be regenerated into various desirable forms for specific applications and therefore has been used in the preparations of thin films in tissue engineering for cell growth. In the present work, silk fibroin was extracted by degumming of silk cocoons and dissolved in ternary solution having CaCl2-ethanol-H2O in 1:2:8 w/v ratios. The obtained fibroin was utilised in the preparation of thin films to study the growth of cells in vivo. Silk solution was utilised in the preparation of thin films to study the growth of cells in vivo. The extracted silk fibroin solution was analysed for protein concentration and presence of fibroin by Lowry’s method and UV-Visible Spectroscopy. SDS-PAGE was performed to confirm the molecular weight of the protein. The antibacterial test was carried out to check the microbial activity. The size of the synthesised fibroin protein was determined by particle size analysis. The surface morphology was studied under Scanning Electron Microscope. Thin films were prepared on a glass slide and characterised under Atomic Force Microscopy. Silk thin films can be used for the generation of tissues and cells as a biological substrate.

 

Keywords : Fibroin, Biomaterial, Silk thin films, Degumming, Ternary solution

 

 

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