Volume No. 10 Issue No.: 4 Page No.: 664-673 April-June 2016

 

STANDARDIZATION OF MEDIA COMPOSITION AND CULTURE CONDITIONS FOR THE GROWTH OF Botrycoccus braunii

 

Shivandappa*, Agrawal P.1 and Krishna V.2

1. R. V. Centre for Cognitive Technologies, R. V. C. E. Campus, R. V. Vidyaniketan Post,Mysore road, Bangalore, Karnataka (INDIA)
2. Department of Biotechnology, Kuvempu University, Shankaraghatta, Shimoga Dist., Karnataka (INDIA)

 

Received on : January 10, 2015

 

ABSTRACT

 

Optimization of the medium for the growth of Botryococcus braunii is an essential for industrial point of view as increase in its biomass is indirectly increases the yield of biodiesel. At present there are many media available, but still improvement in an existing media is a need of the hour and improvements in the contents of existing media directly or indirectly help in achieving optimum growth conditions for B.braunii. Among the media available for the growth of B.braunii, very few media support for elicited production of biomass. Manipulation of media components is one of the approaches to enhance the growth of an Alga, which is indirectly increases the yield of oil content of the algal cells. The efforts were made to enhance the yield of biomass and indirectly increase the oil content by changing the ingredients of existing media. The growth of B.braunii on standard media viz., Chu and MiEB1B12 (Medium with Vitamin B1,B12) and their respective modified media were studied. Both Chu and MiEB1B12 modified and were used to culture B.braunii, there is significant change in yield of biomass. In case of modified Chu medium, concentration of KI, KNO3 and MgSO4.H2O reduced to half of its standard concentration and whereas in case of modified MiEB1B12 medium the concentration of macronutrients was increased two fold of the standard concentration and the concentration of micronutrients reduced to half of its standard concentration. For both modified media the optimum temperature and pH were found to be 32C and 7.9 respectively. And photoperiod in both closed and open systems was also studied, it was found that under artificial (14:10) light it took almost 17 days to get sufficient biomass. Whereas in case of open system culture, it was found that under natural (10:14) light it took just 14 days to get sufficient biomass. With this observation, we formulated standard culture medium and standard conditions for the growth of B.braunii.

 

Keywords : Botryococcus braunii, Media optimization, Algal biomass, Biodiesel production, Open system culture

 

 

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