Volume No. 10 Issue No.: 3 Page No.: 486-491 Jan-Mar 2016

 

MICROPROPAGATION OF ENDANGERED MEDICINAL HERB Chlorophytum borivilianum SANT. ET FERNAND

 

Shrivastava A., Patel A. and Dwivedi S.

1. Department of Botany and Biotechnology, Government Science College, Rewa, Madhya Pradesh (INDIA)
2. Department of Environmental Biology and Biotechnology, Awadhesh Pratap Singh University, Ananthpur, Rewa, Madhya Pradesh (INDIA)

 

Received on : October 15, 2015

 

ABSTRACT

 

Chlorophytum borivilianum Sant. et Fernand an endangered herb is valued for several medicinal properties in its tuberous roots. An efficient and reproducible method for micropagation from explant has been developed. Young shoot buds were used as explants for rapid multiplication of Safed musli (Chlorophytum borivilianum). The explants were cultured into medium containing basal salts of Murashige and Skoog (MS) and various concentrations of 6-benzylaminopurine (BAP) and kinetin (KN) for shoot induction. Treatment containing 2.5 mg/l BAP produced the highest mean number of shoots per explants (14.52) and 2.5 mg/l KN highest mean length of shoots (4.52 cm) after 28 days of culture. Regenerated shoots were successfully rooted on MS medium supplemented with 1.5 mg/l indole-3-butyric acid (IBA) and 30 g/l sucrose. For ex vitro establishment, well-rooted plantlets were transferred in potting medium containing soil : sand (1:1) with VAM.

 

Keywords : Chlorophytum borivilianum, Micro-propagation, Shoot bud, Ex vitro rooting, Bio-hardening

 

 

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