Volume No. 10 Issue No.: 3 Page No.: 423-435 Jan-Mar 2016

 

DECOLORIZATION OF REACTIVE BLACK 5 BY PURE CULTURE OF IE1, C1 AND THEIR MIXTURE

 

Bhuktar J. J. and Manwar A. V.

1. Department of Microbiology, Government Institute of Science, Nipatniranjan Nagar, Aurangabad, Maharashtra (INDIA)
2. Department of Microbiology, Dnyanopasak College, Parbhani, Maharashtra (INDIA)

 

Received on : October 10, 2015

 

ABSTRACT

 

Release of colored textile effluents is undesirable in the aquatic environment as they reduce light penetration, thereby affecting aquatic life and limits utilization of the water media. Microbial bioremediation is an alternative treatment option available other than the commonly employed physicochemical and biological methods to treat these toxic effluents. Two unidentified bacterial cultures were selected for investigation of their ability to degrade RB5 dye in liquid medium. The cultures showed best decolorization under aerobic and shaking conditions at pH 7 and room temperature (282°C). The individual cultures of IE1 and C1 exhibited 71.74% and 73.34% respectively whereas mixture of both showed 89.56% decolorization within 6 h at a dye concentration of 200mg/l, while at higher dye concentration (1000mgl-1 and 5000mgl-1) the decolorization was decreased. The mixture of two culture exhibited decolorization ability (34.83% - 95.24%) over a wide pH range (pH 4.0 to 10.0) compared to individual culture. However, the removal of peptone from the medium inhibited the decolorization activity of the individual cultures whereas their mixture exhibited 15% decolorization of Reactive Black 5 dye. The microbial decolorization of dyes in an indigenously designed fixed film bioreactor showed almost 88% decolorization for individual cultures while 98.34% for their mixture within 48h.

 

Keywords : Azo dye, Bacteria, Decolorization, Bioreactor, Temperature

 

 

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