Volume No. 2 Issue No.: 4 Page No.: 633-638 April-June 2008

 

DELETERIOUS EFFECTS OF CHEMICAL MUTAGENS ON ECONOMICALLY IMPORTANT PLANT Hibiscus sabdariffa VAR. AMV1 LINN.

 

Padma Shrivastava* and H. N. Tiwari

Department of of Botany, Govt. PG College, BHEL, BHOPAL (India)

 

Received on : October 13, 2007

 

ABSTRACT

 

In the present study the chemical mutagens used were ethyl methane sulphonate, N-methyl-N-nitro-N-nitrosoguanidine, di-ethyl sulphate and a physical mutagen represented by gamma radiation. The dry seeds were used for gamma irradiations while dry as well as 6, 12 and 18 hrs. Pre-soaked seeds were utilized for chemical mutagen treatments. The different mutagens utilized in various induced mutability of physical and chemical mutagens are also discussed.
The results obtained in the present investigation are presented under the two major heads M1 and M2 generations. In the first part, the immediate effect of all the mutagens on M1 parameters like germination, seeding height, and survival of plants, chromosomal aberrations, chlorophyll chimeras, pollen sterility and seed set are incorporated.
All these chemical mutagens induced more pollen sterility and seed sterility in economically important plant H. sabdariffa L. var. AMV1.
Mutagen induced genetic changes are shown in the form of pollen sterility in M1 generation. Forese

 

Keywords : Ethylmethane sulphonate, Diethyl sulphate, Floral variations, N-methyl-N-Nitro-N-nitrosoguanidine,

 

 

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