Volume No. 8 Issue No.: 3A Page No.: 568-573 Jan-Mar 2014

 

In vitro CONTROL OF BACTERIAL BLIGHT CAUSING ORGANISM (Xanthomonas xonopodis PV. punicae) OF POMEGRANATE

 

Mahesh Pawar, Manoj Kadam and Bipinraj Kunchiraman Nirichan*

Department of Microbial Biotechnology, Rajiv Gandhi Institute of IT and Biotechnology, Bharati Vidyapeeth Deemed University, Pune (INDIA)

 

Received on : September 20, 2013

 

ABSTRACT

 

Pomegranate cultivation in many part of India has been severely affected by bacterial blight caused by Xanthomonas axonopodis pv. punicae. Extensive agricultural paractice along with large amount of pesticide as well as antibioitc is required to control this diease. In an effort to find an alertnative to the existing chemical contol method studies were carried out to isolate pathogenic bacteria and to screen action of differnet plant extracts against the pathognic isolates. Four bacterial cultures isolated from infected fruit samples were identified as Xanthomonas by biochemical characterization. These cultures produced bacterial blight on tested pomegranate leaf, hence proved to be pathogen. Anti-microbial activity of water extracts of 42 different plants were investigated against these isolates in-vitro. Water extracts of fruits of five plants, Mesua ferrea, Terminalia beleria, Piper nigrum, Emblica officinalis and Terminalia arjun, inhibited all four isolates. MIC of these water extracts ranged between 2.25 to 0.15 mg/ml. This study would be highly useful to control the bacterial blight of pomegranate without costly antibiotic and other pesticides.

 

Keywords : Pomegranate, Bacterial blight, Mesua ferrea, Terminalia beleria, Piper nigrum, Emblica officinalis, Terminalia arjun

 

 

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