Volume No. 8 Issue No.: 3A Page No.: 563-567 Jan-Mar 2014

 

ASSOCIATION OF THE 45 T/G AND 276 G/T POLYMORPHISMS IN THE ADIPONECTIN GENE WITH TYPE 2 DIABETES IN SOUTH INDIAN POPULATION

 

Thirunavukkarasu A.,* Nithya R.,1 Muthukumaran K. and Sivasankari C.2

1. Department of Biotechnology, Govt. College of Technology, Coimbatore, Tamil Nadu (INDIA)
2. Department of Chemistry, Govt. College of Technology, Coimbatore, Tamil Nadu (INDIA)

 

Received on : October 15, 2013

 

ABSTRACT

 

Type 2 diabetes (T2D) is a multifactorial and polygenic disease that involves insulin resistance and impaired glucose induced insulin release. Besides the environmental factors, the influence of genetic changes also drives the susceptibility of the disease. One such candidate gene for T2D is the gene encoding for adiponectin (Acrp30), located on the chromosome 3. The gene product is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Acrp30 polymorphism plays an important role in obesity and type 2 diabetes. But the role of this polymorphism with T2D is not yet studied in Indian population. Hence, the study paying attention to explore the association of adiponectin gene polymorphisms (45T/G and 276G/T) with type 2 diabetes in local population of Thanjavur, India. This study examined the association between the allelic polymorphisms (45T/G and 276G/T) in Acrp30 gene with type 2 diabetes in the local population of Thanjavur. Genotypic analysis was performed on 96 type 2 diabetic patients and age-sex matched non-diabetic individuals using Polymerase Chain Reaction (PCR) based Restriction Fragment Length Polymorphism (RFLP). Chi square analysis (?2) has been made to find out the significant difference in the genotypic distribution among the population. Genotypic correlation was made with phenotypic variables including Body Mass Index (BMI), Waist-to-Hip Ratio (WHR), insulin and adiponectin levels for those polymorphisms of the polymorphisms tested, the significant association with BMI (P<0.05), WHR (P<0.05) adiponectin (P<0.01) and insulin (P<0.01) was observed only for the SNP 276G/T. also it shows significant differences in their genotypic distribution from the results of ?2 analysis. With these evidences, the study supports the hypothesis that 276G/T polymorphism is associated with T2D and it can be considered as a strong marker for Type 2 diabetes.

 

Keywords : Acrp30 gene, Case–control study, Type 2 diabetes, Obesity, South-Indian population

 

 

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