Volume No. 10 Issue No.: 1 Page No.: 73-78 July-September 2015

 

ACUTE TOXICITY EFFECTS OF TiO2 AND Cd(OH)2 NANOPARTICALS (NPS) ON EARTHWORM SPECIES, Eudriles eugeniae

 

More B. C.1 and Patole S. S. *2

1. Department of Zoology, Karm. A. M. Patil Arts, Commerce & Kai. Annasaheb N.K.Patil Science Senior College, Pimpalner Tal. Sakri, Dist. Dhule, Maharashtra (INDIA) 2. Department of Zoology, V. V. Mís. S. G. Patil Arts, Commerce and Science College, Sakri, Dist- Dhule, Maharashtra (INDIA)

 

Received on : April 10, 2015

 

ABSTRACT

 

Nanoparticles (NPs) have wide range of application in the world. Environmental point of view, the safety of NPs in relation to their toxicity is very essential. Present study is under taken to evaluate the acute toxicity effect of NPs on earthworm species Eudrilus eugeniae exposed for 24 h in petriplate containing variable concentration of NPs viz., 0.7, 7.0 and 70 mg /ml. At the end of the experiment antioxidant activity was estimated, the activity of CAT (catalase) was found to be significantly decreased in increasing concentration of both TiO2 and Cd (OH)2 NPs. This depleted catalase activity can suggest increased oxidative stress. Similarly, the activity of MDA was found to be significantly increased in increasing concentration of TiO2 and Cd (OH)2 as compare to control. These increased MDA activity may be indicative of free radical tissue injury. At the same time carbohydrates and protein content was found to decreasing in both the NPs. The bioaccumulation of Ti and Cd was also estimated, cadmium was more bioaccumulated than that of the Ti. Our present study shows that both TiO2 and Cd (OH)2 NPs exert harmful effects to E. eugeniae in their increasing concentration and the toxicity of Cd (OH)2 is higher than TiO2.

 

Keywords : Nanoparticals, Acute toxicity, Antioxidant, Bioaccumulation, Earthworm

 

 

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