Volume No. 10 Issue No.: 1 Page No.: 40-46 July-September 2015

 

MICROPROPAGATION OF ROSE CULTIVARS : BIOTECHNOLOGICAL APPLICATION TO FLORICULTURE

 

Saklani Kumud*, Pant Hem1 and Rawat Vijay2

1. Department of Biotechnology, Shri Guru Ram Rai (PG) College, Pathri Bagh, Dehradun, Uttarakhand (INDIA) 2. Department of Economics, Shri Guru Ram Rai (PG) College, Pathri Bagh, Dehradun, Uttarakhand (INDIA)

 

Received on : February 09, 2015

 

ABSTRACT

 

The nodal explants of rose cultivars were cultured in MS medium supplemented with different concentrations of PGRs for the optimization of growth conditions for shoot proliferation. The best multiplication of nodal explants occurrred in the combination concentration of BAP and KN (5.0 mgl-1+2.5 mgl-1). Half strength MS medium containing NAA and BAP (2.0 mgl-1+0.5 mgl-1) in combination was most effective for rooting. Well rooted microshoots were dipped in bavistin (0.1%, w/v; 2 min), washed with running tap water and transferred to plastic cups containing sand and garden soil (1:1) and showed healthy growth with 60% survival in ex-vitro conditions.

 

Keywords : Rose cultivars, Nodal explants, MS medium, Micropropagation, Acclimatization

 

 

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