Volume No. 10 Issue No.: 1 Page No.: 35-39 July-September 2015

 

PCR-RFLP ANALYSIS OF HA AND NA GENES OF A H5N1 ISOLATE BEING A POSSIBLE ESCAPE MUTANT

 

Bhardwaj Pooja1, R. Sridevi*1, Nagarajan Shanmugasundaram1, Harshad.V.Murugkar1, Shiv ChandraDubey1,Jain Rajlaxmi1and Prakash Anil2

1. High Security Animal Disease Laboratory, Anand Nagar, Bhopal (INDIA) 2. Department of Biotechnology, Barkatullah University, Bhopal (INDIA)

 

Received on : February 10, 2015

 

ABSTRACT

 

New AIV strain continue to emerge because of genetic instability of influenza virus and lack of proof reading and repair mechanism makes AIV genome easily prone to error that occur during replication. This study provide an update of H5N1 being an escape mutant which escaped from a immunized bird in aimmunization experiment conducted on birds with virus strain A/Duck/Tripura/103597/2008(H5N1). This article provide an update of H5N1 virus as it was evolving, creating the potential for new strain that is efficiently transmitted from birds to birds, birds to human or human to human remains highly lethal. The emergence of escape mutant may be either because of antigenic drift or genetic shift which results in H5N1 variants.

 

Keywords : Avian influenza(AIV), H5N1 , PCR -RFLP, HA, NA

 

 

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